Journal: Neuro-Oncology Advances

Article Title: The brain-penetrant cell-cycle inhibitor p28 sensitizes brain metastases to DNA-damaging agents

doi: 10.1093/noajnl/vdad042

Figure Lengend Snippet: p28 crosses the BBB and preferentially localizes to Brain metastases (BMs). (A) Confocal images of the penetration of normal and cancer cells by p28. Human cancer cell lines (MDA-231BR, BCA-1, Mel-7, and A549) and normal cells (MCF-10A and fibroblasts) were cultured with Alexa Fluor 568-labeled p28 at 37°C for 2 hours, and images were obtained by confocal microscopy. Red, p28; blue, DAPI (nucleus). (B) MDA-231BR brain-specific metastatic triple-negative breast cancer, BCA-1 breast cancer, Mel-7 melanoma, or A549 lung cancer cells were injected into the left cardiac ventricle of athymic mice. ICG-labeled p28 was intravenously injected into the mice. Near-infrared fluorescence imaging of the ICG-p28 signal (gray) in coronal brain sections (yellow dotted line on the anterior-dorsal view) of mice injected with MDA-231BR, BCA-1 or Mel-7 cells or in the anterior-dorsal view of the brain of mice injected with A549 cells. H&E staining of brain sections confirmed the presence of BMs (Tu).

Article Snippet: A549 human lung cancer cells, MCF10A, and fibroblasts were purchased from American Type Culture Collection.

Techniques: Cell Culture, Labeling, Confocal Microscopy, Injection, Fluorescence, Imaging, Staining